Summary
Type: SPE
Application #: 302480
Catalog#: 730003 - SPE Cartridges, Chromabond, C18, 3ml, PP, 500mg, 50/pk
Detection: HPLC
Conditions: Column conditioning: The SPE cartridges are solvated with 3 ml of methanol and 2 ml of acetonitrile, then washed with 20 ml of distilled water with special care in order to prevent any air from entering.
Sample application: The solution to be purified (about 10 ml) is placed in the cartridge reservoir and then percolated.
Column washing: The phase is rinsed with 2 ml of distilled water to eliminate most of the polluting substances and dried under aspiration for 1 min.
Elution: with 2 ml methanol/acetonitrile 50:50 (v/v).
Evaporation: The eluate is placed in a 10 ml conic flask and then evaporated to dryness with a rotating evaporator under vacuum (40°C).
Authors: Mabon N Wathelet J-P Marlier M
Source: Talanta 49 (1999) 199-206